Age-related histomorphometrical changes in the cerebellum of autopsied elderly individuals
DOI:
https://doi.org/10.47456/rbps.v24i1.34720Keywords:
Cerebellum, Aging, Purkinje Cells, Granulosas CellsAbstract
Introduction: The aging process occurs in all living beings; however, it is accompanied by the incidence of several histomorphometrical changes. Those age-related changes affect mainly the central nervous system (CNS) organs and above all, the Cerebellum. Objective: to analyze the density of Purkinje cells and the thickness of the granular layer of cerebellum in old and Young-adults, also made an analysis between genders. Methods: We selected 20 autopsied individuals and grouped in: (n=10) young-adults and (n=10) old-adults and anatomically remove fragments from the vermis posterior lobe (CBV) and the right posterior lobe of the cerebellum (CBL). Both fragments proceed to histological process and hematoxylin-eosin stain protocol. Subsequently, with the computational software AxionVision (Zeiss, Germany) and a light microscope, fragments were analyzed in order to obtain density of Purkinje cells and granular layer thickness (μm) by a free interactive system Image J® (National Institutes of Health, Bethesda, EUA). Results: The Old-adults group has showed a tendency of decrease on the Purkinje cells density in both analyzed areas, vermis and lateral lobe, without statistic relevance. The thickness of the granular layer has showed a significant increase on the old-age individuals in both regions. The subjects were also analyzed by gender, showing a significant decrease of the granular layer thickness in the female group on the CBV. Conclusion: The aging process leads to morphological changes in different areas of cerebellum cortex, it is seems that those age-related modifications advance different between the two genders.
Downloads
References
Nordon DG, Guimarães RR, Kozonoe DY, Mancilha VS, Neto VSD. Perda cognitiva em idosos. Rev Fac Ciênc Médicas Sorocaba. 2009;11(3):5-8.
Reuter-Lorenz PA, Jonides J, Smith EE, Hartley A, Miller A, Marshuetz C et al. Age differences in the frontal lateralization of verbal and spatial working memory revealed by PET. J Cogn Neurosci. 2000;12(1):174-87.
Seidler RD, Bernard JA, Burutolu TB, Fling BW, Gordon MT, Gwin JT et al. Motor control and aging: links to age-related brain structural, functional, and biochemical effects. Neurosci Biobehav Rev. 2010;34(5):721-33.
Paul R, Grieve SM, Chaudary B, Gordon N, Lawrence J, Cooper N et al. Relative contributions of the cerebellar vermis and prefrontal lobe volumes on cognitive function across the adult lifespan. Neurobiol Aging. 2009;30(3):457-65.
Gilerovich EG, Fedorova EA, Grigorev IP, Korzhevskii DE. Morphological bases of reorganization of the rat cerebellar cortex in senescence. Zhurnal Evoliutsionnoĭ Biokhimii Fiziol. 2015;51(5):370-6.
Rogers J, Zornetzer SF, Bloom FE, Mervis RE. Senescent microstructural changes in rat cerebellum. Brain Res. 1984;292(1):23-32.
Zhang C, Zhu Q, Hua T. Effects of ageing on dendritic arborizations, dendritic spines and somatic configurations of cerebellar purkinje cells of old cat. Pak J Zool. 2011;43(6):1191-6.
Bernard JA, Seidler RD. Moving forward: age effects on the cerebellum underlie cognitive and motor declines. Neurosci Biobehav Rev. 2014;42:193-207.
MacLullich AMJ, Edmond CL, Ferguson KJ, Wardlaw JM, Starr JM, Seckl JR et al. Size of the neocerebellar vermis is associated with cognition in healthy elderly men. Brain Cogn. 2004;56(3):344-8.
Bernard JA, Leopold DR, Calhoun VD, Mittal VA. Regional cerebellar volume and cognitive function from adolescence to late middle age. Hum Brain Mapp. 2015;36(3):1102-20.
Zhang C, Hua T, Zhu Z, Luo X. Age-related changes of structures in cerebellar cortex of cat. J Biosci. 1 mar 2006;31(1):55-60.
Larsell O. Morphogenesis and evolution of the cerebellum. Arch Neurol Psychiatry. 1934;31(2):373-95.
Aquino Favarato GKN, Silva ACS, Oliveira LF, Fonseca Ferraz ML, Teixeira PA, Cavellani CL. Skin aging in patients with acquired immunodeficiency syndrome. Ann Diagn Pathol. 2020;24:35-9.
Manni E, Petrosini L. A century of cerebellar somatotopy: a debated representation. Nat Rev Neurosci. 2004;5(3):241-9.
Bernard JA, Seidler RD. Relationships between regional cerebellar volume and sensorimotor and cognitive function in young and older Adults. The Cerebellum. 2013;12(5):721-37.
Zhang C, Zhu Q, Hua T. Aging of cerebellar Purkinje cells. Cell Tissue Res. 2010;341(3):341-7.
Sabbatini M, Barili P, Bronzetti E, Zaccheo D, Amenta F. Age-related changes of glial fibrillary acidic protein immunoreactive astrocytes in the rat cerebellar cortex. Mech Ageing Dev. 1999;108(2):165-72.
Raz N, Rodrigue KM. Differential aging of the brain: patterns, cognitive correlates and modifiers. Neurosci Biobehav Rev. 2006;30(6):730-48.
Ampatzis K, Dermon CR. Sex differences in adult cell proliferation within the zebrafish (Danio rerio) cerebellum. Eur J Neurosci. 2007;25(4):1030-40.
Oguro H, Okada K, Yamaguchi S, Kobayashi S. Sex differences in morphology of the brain stem and cerebellum with normal ageing. Neuroradiology. 1998;40(12):788-92.
Luft AR. Patterns of age-related shrinkage in cerebellum and brainstem observed in vivo using three-dimensional MRI volumetry. Cereb Cortex. 1999;9(7):712-21.
Raz N, Dupuis JH, Briggs SD, McGavran C, Acker JD. Differential effects of age and sex on the cerebellar hemispheres and the vermis: A Prospective MR Study. 1998;7.
Torvik A, Torp S, Lindboe CF. Atrophy of the cerebellar vermis in ageing a morphometric and histologic study. :12.
Raz N, Gunning-Dixon F, Head D, Williamson A, Acker JD. Age and sex differences in the cerebellum and the ventral pons: a prospective MR study of healthy adults. 2001;7.
Additional Files
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Brazilian Journal of Health Research
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
