Evaluation of staining techniques in the laboratory diagnosis of cryptosporidiosis

Authors

  • Mariana A. Cruz Universidade Federal de Juiz de Fora. Juiz de Fora/MG, Brasil
  • Thiago R. Santos Universidade Federal de Juiz de Fora. Juiz de Fora/MG, Brasil https://orcid.org/0000-0003-1464-9807
  • Ana Letícia Fonseca Universidade Federal de Juiz de Fora. Juiz de Fora/MG, Brasil https://orcid.org/0000-0001-5558-7193
  • ' Universidade Federal de Juiz de Fora. Juiz de Fora/MG, Brasil
  • Alexandra Menezes Anjos Fundação Hospitalar do Estado de Minas Gerais. Juiz de Fora/MG, Brasil https://orcid.org/0000-0001-7931-3752
  • Ronaldo Rodrigues Costa Fundação Hospitalar do Estado de Minas Gerais. Juiz de Fora/MG, Brasil
  • Marcone Augusto Leal Oliveira Universidade Federal de Juiz de Fora. Juiz de Fora/MG, Brasil https://orcid.org/0000-0001-6026-9608
  • Paula Rocha Chellini Universidade Federal de Juiz de Fora. Juiz de Fora/MG, Brasil
  • Lauren H. Jaeger Universidade Federal de Juiz de Fora https://orcid.org/0000-0001-8031-7742

DOI:

https://doi.org/10.47456/rbps.v25i3.40457

Keywords:

Cryptosporidium spp., Permanent stains, Microscopy, Diagnosis, Quality control

Abstract

Introduction: Cryptosporidiosis is a diarrheal disease that mainly affects children and immunocom­promised individuals. Laboratory diagnosis is based on permanent staining techniques and many challenges still need to be overcome for its implementation as a routine in clinical laboratories. Objec­tives: Compare the effectiveness of different staining techniques in the detection of Cryptosporidium spp. in stool samples and evaluate the best methodology to be implemented in clinical laboratories. Methods: Individuals presenting clinical suspicion of cryptosporidiosis were invited to participate in the study. A total of 18 stool samples (with and without 10% formaldehyde preservative) were ana­lyzed. Five different methodologies were performed: Ziehl-Neelsen (ZN) with and without heating, Safranina (SF) with and without heating and Panoptic Fast. Results: Of the 18 samples analyzed, seven (38.9%) were positive for Cryptosporidium spp. by at least one of the staining techniques used. Sta­tistical agreement was “slight” (Kappa=0.36, p<0.0001). The quality of fixation of the fecal smear on slide and staining showed satisfactory results, both macroscopically and microscopically. Conclusion: The present study highlights the frequency of moderate infection with Cryptosporidium spp. in Juiz de Fora and the need to evaluate the techniques before being implemented in the laboratory routine.

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Additional Files

Published

2023-12-27

How to Cite

1.
Cruz MA, Santos TR, Fonseca AL, ’, Anjos AM, Costa RR, et al. Evaluation of staining techniques in the laboratory diagnosis of cryptosporidiosis. RBPS [Internet]. 2023 Dec. 27 [cited 2024 Jul. 18];25(3):27-33. Available from: https://periodicos.ufes.br/rbps/article/view/40457

Issue

Vol. 25 No. 3 (2023)

Section

Artigos Originais

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