Detection of SARS-CoV-2 in wastewater from a penal establishment and an university hospital: protocols and standardization of collections
DOI:
https://doi.org/10.47456/rbps.v25i3.41091Keywords:
Wastewater-Based Epidemiological Monitoring, Planning, COVID-19 Testing, Problem-Based Learning, Penal institution, Hospitals UniversityAbstract
Introduction: Sewage monitoring as a way to control COVID-19 is recognized worldwide since 2020. The reliability of the results depends on a representative methodology of all the variables involved in the process. The peculiarities of each collection environment make the standardization of procedures an arduous and non-linear process. Objective: This report aims to show the challenges and overcoming in the process of collecting and storing wastewater samples. Methods: collection of sewage samples for physical-chemical and molecular analysis for the detection of SARS-CoV-2, from a semi-open penal establishment and a university hospital. Experience report: The collections were carried out effectively and sent to Biosafety Level 2 laboratories for the initial procedures of pre-filtration and concentration of SARS-CoV-2. The difficulties encountered were: bureaucracy in the process of releasing the project execution, specificity of the pipes, difference in the representativeness of the samples, need for speed in the transport of the samples, unhealthy environments in the physical structures of the evaluated sewage, presenting physical and biological risks for the team. Conclusion: The main aspect of the study was the physical-chemical characterization and monitoring of the genetic material of SARS-CoV-2, but difficulties encountered initially, showed the need for standardization in future wastewater projects.
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